Kuva muri Mutarama 2020 kugeza Ukwakira 2020, ubushakashatsi bw’uburyo bwakorewe mu bitaro bya kaminuza ya Pisa bwasohoye kuri Microbiology ya BMC.Goldstream®Fungus (1-3) -β-D-Glucan Ikizamini cyakoreshejwe mugushakisha urwego rwa BDG uhereye kuri BAL.Ibisubizo byagereranijwe na aByuzuye Automatic Kinetic Tube Umusomyi IGL-200kuva muri Era Biologiya.Ubushakashatsi bwerekana ko BDG igomba kwitabwaho kubera agaciro kayo ko guhanura.Kandi byavuyemo akamaro muguhagarika isuzuma rya PCP kubarwayi bose bagenzura nabi.
Amavu n'amavuko:
Ibipimo bya zahabu yo kwisuzumisha kuriPneumocystis jiroveciiihagarariwe na microscopique visualisation ya fungus iva mu myanya y'ubuhumekero ya kliniki, nk'amazi ya bronchoalveolar-lavage, asobanura “byemejwe”P. jiroveciiumusonga, mu gihe qPCR yemerera gusobanura “bishoboka” kwisuzumisha, kubera ko idashobora kuvangura kwandura ubukoloni.Nyamara, uburyo bwa molekile, nkibihe byanyuma PCR na qPCR, birihuta, byoroshye gukora no gusobanura, bityo bigatuma laboratoire isubiza umuganga amakuru yingirakamaro ya mikorobi mugihe gito.Ubu bushakashatsi bugamije kugereranya microscopi hamwe na molekuline hamwe na beta-D-glucan yo gusuzuma indwara ya bronchoalveolar-lavage ituruka ku barwayi bakekwahoPneumocystis jiroveciiumusonga.Amazi ya Bronchoalveolar-lavage avuye mu byago cumi n'umunani byinshi hamwe n’ibintu bine bigenzura nabi byakorewe metroamine ya metroamine ya Grocott-Gomori, amaherezo ya PCR, RT-PCR, hamwe na beta-D-glucan
Ibisubizo:
Ingero zose za microscopique nziza bronchoalveolar-lavage ntangarugero (50%) nazo zatanze umusaruro ushimishije kumpera yanyuma nigihe nyacyo PCR nibindi byose, ariko bibiri, byavuyemo ibyiza nanone muburyo bwa beta-D-glucan.Iherezo rya PCR na RT-PCR ryagaragaje 10 (55%) na 11 (61%) kuri 18 by'icyitegererezo, bityo bikerekana ibyiyumvo byiyongereye ugereranije na microscopi.RT-PCR yose hamwe na Ct <27 byemejwe mikorosikopi, mugihe ingero zifite Ct≥ 27 zitari zo.
Umwanzuro:
Akazi kacu karerekana ko ari ngombwa kuvugurura no gusobanura uruhare rwo gusuzuma indwara ya molekuline mu mavuriro yihariye, nkaP. jiroveciikwandura, ni ibintu bidasanzwe ariko nanone bikabije kandi byihuta byiterambere byubuvuzi bigira ingaruka kubakira badafite ubudahangarwa byagirira akamaro cyane kwisuzumisha vuba.Abarwayi batoranijwe cyane, ukurikije ibipimo byo kubishyiramo, bivamo ingaruka mbi muburyo bwa molekile zishobora kuvahoP. jiroveciiumusonga.
Franconi I, Leonildi A, Erra G, n'abandi.Kugereranya uburyo butandukanye bwa mikorobe yo gusuzuma indwara ya pneumocystis jirovecii umusonga ku mazi ya bronchoalveolar-lavage.BMC Microbiol.2022; 22 (1): 143.Yatangajwe 2022 Gicurasi 21. doi: 10.1186 / s12866-022-02559-1
Igihe cyo kohereza: Jun-14-2022